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    • 专利摘要:
    Device and procedure for the simultaneous lyophilization of a plurality of biological samples guaranteeing the traceability of said samples destined to be housed inside some coded containers (1), the device comprises a diffuser block (2) that comprises a plurality of openings that define receptacles (21) to house the coded containers (1), where the diffuser block (2) also comprises a perimeter recess (24) configured so that the alloy that surrounds each receptacle (21) is similar in all containers (1) Coded allowing high thermal homogeneity, and the receptacles (21) have through openings defining an open bottom (23) to scan the coded containers (1). Likewise, the device also comprises a transfer cover (3) to guarantee the position of each container (1) when it is transferred to the diffuser block (1) and back to the starting box (4). (Machine-translation by Google Translate, not legally binding)
    公开号:ES2802149A1
    申请号:ES201930619
    申请日:2019-07-04
    公开日:2021-01-15
    发明作者:Montero Andrés Celestino García；De Matos Correia E Vale Alberto Orfao；Palomo Francisco Javier García；Faria Catia Daniela Quintas；Martín Roberto José García；Caro María Pérez
    申请人:Universidad de Salamanca；
    IPC主号:
    专利说明:

    [0001] DEVICE AND PROCEDURE FOR SIMULTANEOUS LYOPHILIZATION OF
    [0003] OBJECT OF THE INVENTION
    [0005] The present invention provides a method and device for the simultaneous lyophilization of multiple biological samples housed within coded containers. More specifically, the present invention describes the combined use of a diffuser block with an open bottom and perimeter emptying that presents a high thermal performance, and a transfer cover designed to transfer the coded containers with the biological samples between different supports to the block. diffuser allowing storage at room temperature guaranteeing at all times the traceability of all lyophilized samples.
    [0007] BACKGROUND OF THE INVENTION
    [0009] At present, samples of tissues, cells, different cellular components, body fluids or culture derivatives, are usually stored in cryogenic containers that require high energy consumption and at the same time a high investment in infrastructure, such as:
    [0010] - mechanical freezing equipment between -20 ° C and -150 ° C or storage systems in liquefied gases (eg liquid N 2 );
    [0011] - Support equipment to avoid / control possible failures (backup equipment -backup-, continuous temperature registers, alarms, autonomous electric generators);
    [0012] - Large spaces necessary to house the freezing equipment, which also requires: i) resistant floors to support the weight of the equipment; ii) air conditioning systems to avoid excessive heating of the rooms; iii) ventilation and forced extraction systems to avoid risks associated with the use of high pressure liquefied gases for backup (CO 2 and / or N 2 ).
    [0014] Therefore, adapting the method of drying samples by lyophilization for storage at room temperature reduces costs, while allowing a greater stability of its quality by not being subject to possible fluctuations in the cold chain, advantages that can be extended to the transport of samples in conditions of extreme ambient temperature.
    [0016] The conservation process by lyophilization is based on the dehydration of the samples until reaching a water content of less than 1.5% (anhydrobiosis) to leave them in an inert and stable state that allows them to be stored at room temperature, guaranteeing their quality for prolonged periods of time. This process is carried out in three consecutive steps:
    [0018] 1) frozen,
    [0019] 2) primary drying or sublimation,
    [0020] 3) secondary drying or desorption.
    [0022] This technology is known and widely used in many processes for preserving products at room temperature and is commonly used in the pharmaceutical, cosmetic and dietetic industries for the preservation of medicines, vaccines, vitamins, food and food supplements. The equipment and techniques used have a high degree of development / complexity and usually use containers to contain the samples to be lyophilized, which have a high surface / volume ratio to facilitate the processing of the samples. However, there is no multi-well diffuser thermal block system on the market that allows the simultaneous freeze-drying of multiple samples, contained in individual containers identified with unique codes, which guarantees both the traceability of the samples and the exact control during the process. and homogeneous temperature in all tubes, regardless of the relative position with respect to the rest of the samples and that can be applied to tubes with relatively low surface / volume ratios.
    [0024] The only diffuser block prototype known to the applicant is that of the company Virtix ("New products: Well Freeze-Drying System", Science, 2008; 320 (5878): 954; and Patel SM & Pikal MJ. "Virtix 96 well freeze-drying system. ”AAPS PharmSciTech, 2011; 12 (1): 372 378) but this equipment does not allow individual identification of each of the samples nor does it guarantee, therefore, their traceability during the freeze-drying process.
    [0025] DESCRIPTION OF THE INVENTION
    [0027] The present invention aims to solve some of the problems mentioned in the state of the art. More specifically, in a first aspect the present invention describes a device for the simultaneous lyophilization of multiple samples based on an open bottom diffuser block, with a perimeter groove, which has a high thermal performance and also comprises a transfer cover. Complementary for the transfer of the samples to other supports thus guaranteeing the traceability at all times of the lyophilized samples.
    [0029] More in particular, a first aspect of the present invention discloses a device for the simultaneous lyophilization of a plurality of biological samples housed within coded containers, comprising a diffuser block of a metal alloy with high thermal conductivity comprising a plurality of openings that define receptacles to house the coded containers and also comprising:
    [0030] - a perimeter void configured so that the alloy that surrounds each receptacle is similar in all coded containers allowing high thermal homogeneity, and
    [0031] - The receptacles have through openings defining an open bottom.
    [0033] Each of the receptacles of the diffuser block encompasses each of the coded containers, individually and enables the transfer of energy by conduction from the lyophilizer plate to the sample container receptacle, as in the vacuum situation in which lyophilization is carried out there is no possibility of transfer by convection.
    [0035] The perimeter emptying of the diffuser block, around the outer receptacles of the diffuser block, causes the amount of metallic alloy that surrounds each sample container receptacle (both in those located in central positions of the diffuser block and in those located in lateral and perimeter) is similar, which translates into a great thermal homogeneity of the set with minimal temperature differences between the receptacles, regardless of the relative position in which each one is in relation to the rest (centered vs. lateral) and the area and volume of metal alloy existing between them.
    [0036] Likewise, the emptying of the base of the diffuser block in each of the receptacles, in addition to allowing direct contact of the sample container with the hot plate of the lyophilizer, enables the simultaneous reading of the coding of the sample containers by means of a system. multiple reading (scanner) of identifier codes (eg two-dimensional or one-dimensional codes).
    [0038] Preferably, the receptacles have a matrix-like configuration with a plurality of rows and columns. That is, the design of the diffuser block can be of multiple matrix configurations, depending on the arrangement and number of receptacles, such as: i) 384 (24x16) matrix; ii) 96 (12x8) matrix; iii) 48 (8x6) matrix; iv) matrix of 24 (6x4); v) 12 (4x3) matrix; vi) 6 (3x2) matrix; vii) matrix of 4 (2X2).
    [0040] Alternatively, the receptacles may have a single row linear type configuration. For example, linear combinations of multiple vessels for example 2,3,4,5,6,7, etc.
    [0042] In said embodiment of the diffuser block with in-line receptacles, the diffuser block may further comprise a lateral recess for the receptacles to allow reading of the coding of the coded containers with the samples by lateral code readers (scanners).
    [0044] Preferably, for an in-line configuration of the receptacles that house the coded sample containers, the diffuser block could contain a relief or groove that only allows it to fit in the position in which the receptacle code is visible from the lateral recess thereof. .
    [0046] The diffuser block can be manufactured in 7075 aluminum-zinc metal alloy (Zicral), although it could also be manufactured in other metal alloys that, while maintaining the high thermal conductivity of the metal alloy, give it lightness and high resistance to traction and fatigue. mechanical, such as alloys with different percentages of aluminum, silver, gold, manganese, magnesium, titanium, silicon, iron, chromium and / or copper.
    [0047] The diffuser block receptacles in any of the configurations (matrix or linear type) are configured to adapt to the different types of coded containers based on:
    [0048] - composition, for example, plastic, metal or glass polymers.
    [0049] - forms, for example, cylindrical, polygonal or with protruding flanges for immobilization in the corresponding receptacle; each one of which can have a flat, concave or differently inclined base or a V-shaped base on all or only some of the slopes.
    [0050] - volume from nanoliters to deciliters.
    [0052] The containers can be hermetically sealed with caps made of a thermoplastic elastomeric polymer (TPE) compatible with the lyophilization process (such as rubber, silicone and / or plastic; or copolymers thereof), arranged on a disposable mesh that allows the simultaneous sealing of all the containers according to the different geometries of the container and the receptacles.
    [0054] The diffuser block with the aforementioned characteristics presents a high thermal conductivity to allow the transfer of energy to the sample containers during the vacuum sublimation phase of the lyophilization process.
    [0056] The tests carried out on the lyophilization equipment showed a heat transfer of up to 30% higher than that produced when the same process was carried out on the starting base plate of the sample containers (made of plastic polymer).
    [0058] In addition, it presents a great homogeneity of temperatures in all the tubes, regardless of their position on the starting plate (centered vs. lateral position), thanks to the perimeter groove and the open bottom.
    [0060] Likewise, the lateral emptying and the open bottom allow the reading of the identification codes of each of the sample containers without having to remove them from the diffuser block, facilitating their identification and guaranteeing their traceability at all times.
    [0062] It allows the orientation of the sample containers, through its unique and unequivocal positioning, within the diffuser block in relation to the system for reading the identification codes of the sample containers (scanner).
    [0063] The device can also comprise a transfer cover that allows the direct and orderly transfer of the sample containers from their original support (eg box) to the diffuser block and their subsequent return to that support for final storage without possibility of positioning error or loss of traceability. It allows, in a simple way, to maintain at all times the relative position of each individual container with respect to the initial position, eliminating possible location errors (traceability) in their transfer.
    [0065] For this functionality, the transfer cover comprises a base where a plurality of columns protrude that form an extension from the base and the combination of each four columns defines a central housing intended to house the coded containers.
    [0067] Likewise, the transfer cover comprises at least one flange on each flank that exceeds the columns in height. Preferably, it comprises a flank on the narrowest flank and two flanks on the longest flank. Additionally, the diffuser block comprises positioning grooves adapted to house the tabs of the transfer cover.
    [0069] The tabs of the transfer cover are also configured to fit into the container starting base (eg commercial box) of the coded containers, facilitating the transfer of said starting base to the transfer cover, and subsequently the cover of transfer to the diffuser block, without losing the traceability of the samples.
    [0071] The combination of the transfer cover with the diffuser block guarantees the traceability of the samples at all times. By allowing the movement of the coded containers containing the samples between the container starting base (eg commercial box) to the diffuser block, consequently, possible errors in the identification of the samples (loss of traceability) are eliminated.
    [0073] The transfer lid can have an intuitive and unique orientation to avoid confusion during the transfer process of samples housed in the coded containers. Furthermore, as mentioned, it is adaptable to the diffuser block and the starting box where the sample containers are housed.
    [0074] The use of this lid can be manual, taking advantage of its geometry to carry out the transfer and transfer between supports, or it can be carried out using a robotic arm.
    [0076] Preferably, the lyophilization installation comprises the device described above with any of its possible variants, as well as further comprising a starting box where the coded containers are initially housed, a lyophilization device configured for freezing and vacuum drying the samples, a lyophilizer tray, a scanner, and a vacuum sealing equipment.
    [0078] In a second aspect of the invention, a method of using the lyophilization installation is disclosed, in which the method comprises:
    [0079] - place the transfer cover on top of the starting box comprising the coded containers forming a set comprising the starting box and the transfer cover,
    [0080] - turn the assembly 180 ° so that the tubes are placed between the columns of the transfer cover and remove the starting box,
    [0081] - place diffuser block so that the tabs of the transfer cover fit into the slots of the diffuser block forming a second set of transfer cover and diffuser block,
    [0082] - turn the second set 180 ° so that the coded containers are placed in the diffuser block,
    [0083] - place the diffuser block oriented in the scanner, proceed to read the codes of the coded containers and store the information generated in a database,
    [0084] - Dispense the biological sample and a lyophilization matrix solution into each coded container,
    [0085] - place the diffuser block on the lyophilizer tray and start lyophilization in the lyophilizer device,
    [0087] The lyophilized biological samples in the coded containers can be transferred back to the starting box following the reverse order of steps so that they maintain exactly the same order and not lose traceability.
    [0088] Subsequently, preferably said starting box, comprising the coded containers with the lyophilized biological samples, is vacuum sealed with the installation's vacuum sealing equipment.
    [0090] The method described above, as well as any of its steps, and the dispensing of the biological samples and the lyophilization matrix solution can be executed by a robot arm.
    [0092] Biological samples that can be used for this process, among others, include: tissues, cells, blood, plasma, serum, cerebrospinal fluid, synovial fluid, amniotic fluid, vitreous humor, aqueous humor, tears, saliva, urine, feces, sweat , semen, cells, exosomes, subcellular organelles, nucleic acids (for example DNA and RNA), drugs, vaccines, toxins, vitamins, enzymes, cofactors, lipids, hormones, peptides, fluorochromes, cofactors, proteins, antibodies, antigens or cytokines.
    [0094] Preferably, the lyophilization matrix is an aqueous solution comprising sugars, surfactants, antioxidants, salts, or combinations thereof.
    [0096] Sugars can be selected from a list comprising: mannitol, sucrose, trehalose, glucose, and combinations thereof; surfactants are selected from the list comprising: polysorbate 20, polysorbate 80, or combinations thereof; the antioxidants comprise epigallocatechin gallate and the salts are selected from the list comprising: TrisCIH, sodium acetate, sodium phosphate, or combinations thereof.
    [0098] EXAMPLES
    [0100] The following application examples serve to illustrate the procedure, but do not limit the scope of the patent. Example of application model for lyophilization of DNA samples:
    [0102] Diffuser block made of 7075 aluminum-zinc metal alloy with a polyhedral shape (127mm long x 85mm wide x 15mm high), with two 12mm recesses at 45 ° in the
    [0103] two corners of a long side (leaving the short sides 77mm wide) and 96 cylindrical shaped receptacles (8mm diameter x 14mm height, plus 1mm end in which its diameter is reduced to 6mm) that go beyond the total height ( 15mm) from the block. The receptacles are arranged in a matrix format with 12 columns x 8 rows, equidistant 1mm at the top and 3.18mm at the bottom. The block has, at its base, a perimeter groove 8mm deep x 4mm wide, separated by 1.5mm from the outside on the long side and 4mm on the short side, which surrounds the entire block, except for a septum of 1, 5mm in the middle of the short left side. At the top, the block has a through slot (15mm deep) 6mm wide x 35mm long, located in the middle of the short right side. In the middle of the two long sides there are two other 2.2mm wide x 14mm long through slots. Along the entire edge of the upper part, the block has a recess 2mm wide and 3mm deep that allows the fitting of the specially designed transfer cover.
    [0105] 96 polypropylene tubes of 0.75ml volume with V-bottom and precoded at their base with 2D codes, organized in their starting box (Loborack-96 V-botton of 0.75ml; Micronic).
    [0107] Transfer cover made of ABS plastic polymer with 2mm wide walls and polyhedral shape (127mm long x 85mm wide x 34mm high). It has 117 cylindrical columns of 19mm high x 3mm in diameter, organized in a matrix of 13 columns x 9 rows, which leave between them 96 positions of 9mm in diameter organized in a matrix of 12 columns x 8 rows. On the short right side there is a 24mm long x 2.5mm wide x 10mm deep flange that protrudes 2mm over the side edge. In the middle of the two long sides there are two other smaller tabs measuring 1.8mm wide x 13mm long that protrude 9mm over the side edges of the lid.
    [0109] Lyophilization matrix composed of 0.21M Trehalose, dissolved in 10mM Tris-HCl 1mM EDTA buffer, pH 8.0 (1x TE buffer). Boxes of 2D pre-coded polypropylene tubes, Loborack-96 V-botton 0.75ml (Micronic). TPE Lyo Caps-96 (Micronic) lyophilization caps.
    [0111] Epsilon 2-4 LSC-plus lyophilizer equipment (Martin Christ, Germany) and liquid handling robot, with integrated barcode scanner, (mod. Tecan EVO150; Tecan, Switzerland). 2D tube scanner (Micronic, Lelystad, the Netherlands). Vacuum Bag Sealing Equipment
    [0113] 1. - Preparation of DNA samples to be lyophilized.
    [0114] DNA samples must be at room temperature (20-25 ° C), or refrigerated at 4 ° C, prior to starting the process.
    [0116] 2. - Transfer of the 2D tubes from the original box to the diffuser block.
    [0117] - The transfer cover is placed on top of the starting tube box.
    [0118] - It is turned 180 ° so that the tubes are, upside down, on the lid and the original box of the tubes is removed.
    [0119] - The diffuser block fits, upside down, on the tubes.
    [0120] - The whole assembly is turned 180 ° again and the transfer cover is removed, so that the 2D tubes are placed on the diffuser block.
    [0122] 3. - Reading the codes of the 2D tubes in the thermoblock
    [0123] The diffuser block oriented in the scanner is placed (there is only one possible positioning) and the codes of the 2D tubes are read with the scanner and the information generated on the identification of each of the 2D codes is kept in a file of data.
    [0125] 4. - Preparation of the DNA sample to be lyophilized
    [0126] Through the robotic arm of the equipment, each of the tubes contained in the 96 receptacles of the diffuser block will be dispensed:
    [0127] - 200ul of DNA at a concentration of 100ng / pl in 1x TE.
    [0128] - 100pl of the 0.21M trehalose matrix in 1x TE.
    [0130] 5. - Freeze-drying process of DNA samples
    [0131] i. The blanket with the 96 lyophilization caps (TPE Lyo Caps-96) is positioned on the 96 tubes with the mixture of DNA and matrix, without pressing to prevent them from closing.
    [0132] ii. The diffuser block with lyophilization tubes and caps are transferred to the lyophilizer tray.
    [0134] Ii. In the lyophilization equipment, the following sequence of lyophilization temperatures, pressures and times is programmed:
    [0135] Atmospheric pressure freezing:
    [0136] Freezing ramp 1 ° C / min from room temperature to -30oC.
    [0137] Stationary phase: 3h at -30oC.
    [0139] Primary drying at 0.380 mbar pressure:
    [0140] Ramp from -30oC to -20 ° C; 1 ° C / min.
    [0141] Stationary phase: 10h at -20 ° C.
    [0142] Ramp from -20 ° C to -10 ° C; 1 ° C / min.
    [0143] Stationary phase: 2h at -10 ° C
    [0145] Final drying at 0.001 mbar pressure:
    [0146] Ramp from -10 ° C to 20 ° C; 0.4 ° C / min.
    [0147] Stationary phase: 4h at 20 ° C.
    [0149] iv. Once the entire lyophilization process is finished, while the equipment is still under vacuum, the tubes containing the samples are closed using the press of the lyophilizer equipment.
    [0151] 6. - Repositioning the tubes in the original container.
    [0152] The 2D tubes are transferred from the diffuser thermal block to the starting box using the tube transfer cover, following the reverse order to that indicated in section 2 of the example.
    [0154] 7. - Final storage of lyophilized products.
    [0155] 1. By means of the equipment of sealing to the empty one proceeds to the final packaging of each one of the boxes of tubes.
    [0156] 2. Each box is stored in the room temperature warehouse designated for this purpose.
    [0158] DESCRIPTION OF THE DRAWINGS
    [0160] To complement the description that is being made and in order to help a better understanding of the characteristics of the invention, according to a preferred example of a practical embodiment thereof, a set of drawings is attached as an integral part of said description. where, for illustrative and non-limiting purposes, the following has been represented:
    [0161] Figure 1.- Shows three perspective views of a preferred embodiment of the diffuser block where the perimeter recess, the open bottom and the coded containers housed in the receptacles of the diffuser block are shown.
    [0163] Figure 2.- Shows three perspective views of the preferred embodiment of the transfer cover where the columns of the transfer cover are shown, as well as the tabs that allow the union between the transfer cover and the diffuser block in a certain and only position.
    [0165] Figure 3.- Shows a perspective view of the diffuser block with the coded containers housed in the receptacles and their positioning in the scanner.
    [0167] Figure 4.- Shows a perspective view of a preferred embodiment, where some of the steps of the method are shown to maintain the traceability of the samples.
    [0169] Figure 5a.- Shows a perspective view of a second alternative embodiment of the diffuser block, with a linear configuration of a row where a view from the upper face is shown and the lateral emptying is represented.
    [0171] Figure 5b.- Shows a perspective view of the second alternative embodiment where it shows the lower face and the perimeter recess, the open bottom and the relief of the receptacles are represented.
    [0173] PREFERRED EMBODIMENT OF THE INVENTION
    [0175] Figure 1 clearly shows a preferred embodiment of a first aspect of the invention, where a diffuser block (2) is shown as a device for the simultaneous lyophilization of a plurality of biological samples destined to be housed inside some coded containers (1) .
    [0177] Figure 1 also shows that the diffuser block (2) has a plurality of openings that define receptacles (21) to house the coded containers (1) therein. Said diffuser block (2) in the preferred embodiment described is made of a metal alloy with high thermal conductivity and has a perimeter recess (22) configured so that the alloy that surrounds each receptacle (21) is similar in all the containers (1) coded allowing a high thermal homogeneity. The diffuser block (2) is also shown to comprise an open bottom (23) since the receptacles (21) have through openings. Furthermore, it is also shown that the diffuser block (2) comprises three transfer slots (24), one on the narrow side of the diffuser block (2) and the other two remaining on the longest side of the diffuser block (2).
    [0179] The diffuser block (2) with the aforementioned characteristics has a high thermal conductivity to allow the transfer of energy to the sample containers during the vacuum sublimation phase of the lyophilization process.
    [0181] Further. It presents a great homogeneity of temperatures in all the coded containers (1), regardless of their position on the starting plate (centered vs. lateral position), thanks to the perimeter groove and the open bottom.
    [0183] Likewise, the open bottom allows the reading of the identification codes of each of the sample containers without having to remove them from the diffuser block, facilitating their identification and guaranteeing their traceability at all times.
    [0185] Figure 2 shows the preferred embodiment described where the device also comprises a transfer cover (3) comprising a base (31) where a plurality of columns (32) protrude, configured in arrangement and quantity, to the plurality of receptacles (21) of the diffuser block (2) adapted to house the coded containers (1).
    [0187] Also shown in Figure 2 is a flange (33) that exceeds the columns (32) in the transfer cover (3) in height. Said flanges (33) are adapted to be housed in the grooves (24) of the diffuser block to allow the junction between the transfer cover (3) and the diffuser block (2) in a determined and unique position. In the preferred embodiment, the transfer cover (3) is made of plastic.
    [0189] Figure 3 shows a perspective view of the diffuser block (2) with the containers (1) coded in the receptacles (21), being scanned in a scanner (7), of a lyophilization facility that comprises a box (4) starting point shown in figure 4, where the coded containers (1) are initially housed, a lyophilization device (not shown) configured for freezing and vacuum drying of the samples, a lyophilizer tray (not shown), a scanner (7), and a vacuum sealing equipment (not shown).
    [0191] Figure 4 shows a perspective view of a second aspect of the present invention, where a method of using the device is shown to guarantee the traceability of the samples in a lyophilization installation that improves the thermal homogeneity of the samples.
    [0193] Initially, the starting box (4) is shown with the coded containers (1), the transfer cover (3), and the diffuser block (2). In a first step of the preferred embodiment described, figure 4 shows that the transfer cover (3) must be placed on top of the starting box (4) comprising the coded containers (1) forming a set (5) comprising the starting box (4) and the transfer cover (3),
    [0195] Subsequently, the assembly (5) must be rotated 180 ° so that the coded containers (1) enter the columns (32) of the transfer cover (3) and remove the starting box (4).
    [0197] Next, it shows that the diffuser block (2) must be placed so that the tabs (33) of the transfer cover (3) fit into the slots (24) of the diffuser block (2), forming a second set (6) of transfer cover (3) and diffuser block (2),
    [0199] The next thing is to turn the second set (6) 180 ° so that the coded containers are placed in the diffuser block (2). Next, the diffuser block (2) is placed oriented in the scanner (7), and the codes of the coded containers (1) are read, allowing the information generated to be stored in a database.
    [0201] In a preferred embodiment, the biological sample and a lyophilization matrix solution are dispensed into each encoded container (1) by means of a robot arm.
    [0203] In this way, the traceability of the samples has been guaranteed, and it is guaranteed in the same way if the same process is done in reverse after lyophilization. Consequently, the diffuser block (2) is placed in the lyophilizer tray and lyophilization is started in the lyophilizer device. Once the samples have been lyophilized, the reverse order is followed to transfer the samples to the starting box (4) maintaining traceability at all times, therefore using the transfer cover (3) again.
    [0204] Finally, the samples already lyophilized in the containers (1) coded in the starting box (4) are vacuum sealed in the starting box (4).
    权利要求:
    Claims (18)
    [1]
    1. - Device for the simultaneous lyophilization of a plurality of biological samples destined to be housed inside coded containers (1), comprising a diffuser block (2) of a metal alloy with high thermal conductivity that comprises a plurality of receptacles ( 21) to house the coded containers (1), characterized in that said diffuser block (2) further comprises:
    - a perimeter drain (22) allowing high thermal homogeneity, and
    - the receptacles (21) have through-holes defining an open bottom (23),
    [2]
    2. - Device for lyophilization according to claim 1, characterized in that the receptacles (21) have a matrix-type configuration with a plurality of rows and columns.
    [3]
    3. - Device for lyophilization according to claim 1, characterized in that the receptacles (21) have a linear configuration of a single row.
    [4]
    4. - Device for lyophilization according to claim 3, characterized in that the diffuser block (2) comprises a lateral recess (25) intended to allow the reading of the coded containers (1) by means of a scanner.
    [5]
    5. - Device for lyophilization according to claim 4, characterized in that the receptacles (21) have a relief (26) configured to allow the coded containers (1) to fit in a single position that allows scanning from the lateral emptying diffuser block (2).
    [6]
    6. - Device for lyophilization according to claim 1, characterized in that it also comprises a transfer lid (3) comprising a base (31) where a plurality of columns (32) protrude in arrangement and quantity coincident with the plurality of receptacles (21) of the diffuser block (2) to house the coded containers (1).
    [7]
    7. - Device for lyophilization according to claim 6, characterized in that the transfer cover (3) comprises at least one flange (33) that exceeds the columns (32) in height and the diffuser block (2) comprises at least one transfer slot (24) adapted to house the tabs (33) of the transfer cover (3) to allow the union between the transfer cover (3) and the diffuser block (2) in a specific and unique position.
    [8]
    8. - Device for lyophilization according to claim 6, characterized in that the transfer cover (3) is made of plastic.
    [9]
    9. - Device for lyophilization according to claim 6, characterized in that the transfer cover (3) is made of a metal alloy,
    [10]
    10. - Device for lyophilization according to claim 1, characterized in that the diffuser block (2) is a 7075 aluminum-zinc metal alloy.
    [11]
    11. - Installation comprising the device described in any one of claims 1-10, comprising a starting box (4) where the coded containers (1) are initially housed, a lyophilization device configured for freezing and vacuum drying of the samples, a lyophilizer tray, a scanner (7), and a vacuum sealing equipment.
    [12]
    12. - Method of use of the facility of claim 11 for lyophilization and traceability of biological samples, characterized in that it comprises:
    A. place the transfer cover on top of the starting box (4) comprising the coded containers (1) forming a first set (5) comprising the starting box (4) and the transfer cover (3),
    B. turn the assembly (5) 180 ° so that the coded containers (1) enter between the columns (32) of the transfer cover and remove the starting box (4),
    C. Position the diffuser block (2) so that the tabs (33) of the transfer cover (3) fit into the slots (24) of the diffuser block (2) forming a second set (6) of the transfer cover ( 3) and diffuser block (2),
    D. turn the second set (6) 180 ° so that the coded containers (1) are placed in the diffuser block (2),
    E. place the diffuser block (2) oriented in the scanner (7), proceed to read the codes of the coded containers and store the information generated in a database, F. dispense the biological sample in each coded container (1) and a freeze-drying matrix solution,
    G. place the diffuser block (2) on the lyophilizer tray and start lyophilization in the lyophilizer device,
    [13]
    13. - Method of use of the facility according to claim 12, characterized in that it comprises transferring the containers (1) encoded with the biological samples and the lyophilization matrix solution after lyophilization to the starting box (4) following the reverse order of steps of claim 12.
    [14]
    14. - Method of use of the facility according to claim 12, characterized in that it comprises vacuum sealing the starting box (4) with the coded containers with the lyophilized samples.
    [15]
    15. - Method of use of the facility according to any of claims 12 or 13, characterized by at least one of the stages A-F is executed by a robotic arm.
    [16]
    16. - Method of use of the facility according to claims 12, characterized in that the biological samples comprise at least one product selected from: tissues, cells, blood, plasma, serum, cerebrospinal fluid, synovial fluid, amniotic fluid, vitreous humor, aqueous humor, tears, saliva, urine, feces, sweat, semen, cells, exosomes, subcellular organelles, nucleic acids (for example DNA and RNA), drugs, vaccines, toxins, vitamins, enzymes, cofactors, lipids, hormones, peptides, fluorochromes, cofactors, proteins, antibodies, antigens, and cytokines.
    [17]
    17. - Method of use of the facility according to claim 12, characterized in that the lyophilization matrix is an aqueous solution that comprises sugars, surfactants, antioxidants, salts or combinations thereof.
    [18]
    18. - Method of use of the facility according to claim 17, characterized in that the sugars are selected from the list that comprises mannitol, sucrose, trehalose, glucose and combinations thereof; surfactants are selected from the list comprising: polysorbate 20, polysorbate 80, or combinations thereof; the antioxidants comprise epigallocatechin gallate and the salts are selected from the list comprising: TrisCIH, sodium acetate, sodium phosphate, or combinations thereof.
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    同族专利:
    公开号 | 公开日
    WO2021001589A4|2021-01-28|
    WO2021001589A1|2021-01-07|
    ES2802149B2|2022-01-11|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US6517526B1|2000-12-29|2003-02-11|Yehuda Tamari|Container for lyophilizing biological products|
    WO2003006904A1|2001-07-09|2003-01-23|Perera, Horacio, Eduardo|Apparatus and process for freezing produce|
    EP2745064A2|2011-09-06|2014-06-25|Rheavita B.V.|Method and system for freeze-drying injectable compositions, in particular pharmaceutical compositions|
    WO2017137637A2|2016-08-05|2017-08-17|Bachem Holding Ag|Drying container|
    US20080175757A1|2007-01-19|2008-07-24|Andrew Powell|Microarray device with elastomeric well structure|
    EP3761001A1|2011-05-18|2021-01-06|Biocision, LLC|Ventilation assisted passive cell freezing device|
    EP2848882B1|2012-05-03|2016-03-09|Schott AG|Method and device for crimping containers for the storage of substances for medical, pharmaceutical or cosmetic uses|
    DE102013112167A1|2013-11-05|2015-05-07|Schott Ag|Support structure for simultaneously holding a plurality of containers for substances for medical, pharmaceutical or cosmetic applications as well as transport and packaging containers with self and method|
    UY37112A|2016-02-05|2017-07-31|Tolmar Therapeutics Inc|VENTILATED COATING PLATE FOR A SYRINGE SET|
    法律状态:
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    优先权:
    申请号 | 申请日 | 专利标题
    ES201930619A|ES2802149B2|2019-07-04|2019-07-04|DEVICE AND PROCEDURE FOR THE SIMULTANEOUS LYOPHILIZATION OF A PLURALITY OF BIOLOGICAL SAMPLES|ES201930619A| ES2802149B2|2019-07-04|2019-07-04|DEVICE AND PROCEDURE FOR THE SIMULTANEOUS LYOPHILIZATION OF A PLURALITY OF BIOLOGICAL SAMPLES|
    PCT/ES2020/070428| WO2021001589A1|2019-07-04|2020-07-02|Device and method for simultaneous lyophilisation of a plurality of biological samples|
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